PDBe 1yar

X-ray diffraction
1.9Å resolution

Structure of Archeabacterial 20S proteasome mutant D9S- PA26 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 42-mer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Proteasome subunit beta Chains: H, I, J, K, L, M, N
Molecule details ›
Chains: H, I, J, K, L, M, N
Length: 217 amino acids
Theoretical weight: 24 KDa
Source organism: Thermoplasma acidophilum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28061 (Residues: 1-211; Coverage: 100%)
Gene names: Ta0612, psmB
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 233 amino acids
Theoretical weight: 25.8 KDa
Source organism: Thermoplasma acidophilum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25156 (Residues: 1-233; Coverage: 100%)
Gene names: Ta1288, psmA
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome activator protein PA26 Chains: O, P, Q, R, S, T, U
Molecule details ›
Chains: O, P, Q, R, S, T, U
Length: 237 amino acids
Theoretical weight: 26.09 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9U8G2 (Residues: 2-231; Coverage: 100%)
Sequence domains: Proteasome activator pa28 beta subunit
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 254.874Å b: 127.512Å c: 181.182Å
α: 90° β: 92.45° γ: 90°
R-values:
R R work R free
0.182 0.182 0.216
Expression system: Escherichia coli BL21(DE3)