› 1y8k

X-ray diffraction
2.3Å resolution

Horse methemoglobin low salt, PH 7.0 (88% relative humidity)

Released:
DOI: 10.2210/pdb1y8k/pdb
PDB entry 1y8k coloured by chain, front view.
PDB entry 1y8k coloured by chain, side view.
PDB entry 1y8k coloured by chain, top view.
Source organism: Equus caballus
Primary publication:
A new relaxed state in horse methemoglobin characterized by crystallographic studies.
Sankaranarayanan R, Biswal BK, Vijayan M
Proteins 60 547-51 (2005)
PMID: 15887226

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135379 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hemoglobin subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 141 amino acids
Theoretical weight: 15.14 KDa
Source organism: Equus caballus
UniProt:
  • Canonical: P01958 (Residues: 2-142; Coverage: 99%)
Gene name: HBA
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 146 amino acids
Theoretical weight: 16.03 KDa
Source organism: Equus caballus
UniProt:
  • Canonical: P02062 (Residues: 1-146; Coverage: 100%)
Gene name: HBB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

1 bound ligand:
Ligand HEM 4 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 62.519Å b: 80.14Å c: 111.315Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.183 0.228

Quick links

Citations

1 citations in other articles

An asymmetric dimer of beta-lactoglobulin in a low humidity crystal form--structural changes that accompany partial dehydration and protein action.
Vijayalakshmi et al. (2008)

3 mentions without citation

Exploiting structure similarity in refinement: automated NCS and target-structure restraints in BUSTER.
Smart et al. (2012)
2 more