PDBe 1y7a

X-ray diffraction
1.77Å resolution

Structure of D153H/K328W E. coli alkaline phosphatase in presence of cobalt at 1.77 A resolution


Function and Biology Details

Reaction catalysed:
A phosphate monoester + H(2)O = an alcohol + phosphate. 
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Alkaline phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 449 amino acids
Theoretical weight: 47.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
  • Canonical: P00634 (Residues: 23-471; Coverage: 100%)
Gene names: JW0374, b0383, phoA
Sequence domains: Alkaline phosphatase
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: I222
Unit cell:
a: 76.208Å b: 164.564Å c: 192.636Å
α: 90° β: 90° γ: 90°
R R work R free
0.205 0.205 0.238
Expression system: Escherichia coli