PDBe 1y0v

X-ray diffraction
3.6Å resolution

Crystal structure of anthrax edema factor (EF) in complex with calmodulin and pyrophosphate

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-2 A Chains: H, I, J, K, L, M
Molecule details ›
Chains: H, I, J, K, L, M
Length: 146 amino acids
Theoretical weight: 16.44 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0DP34 (Residues: 6-149; Coverage: 97%)
Gene name: calm2-a
Structure domains: EF-hand
Calmodulin-sensitive adenylate cyclase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 777 amino acids
Theoretical weight: 90.13 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P40136 (Residues: 33-800; Coverage: 100%)
Gene names: BXA0141, GBAA_pXO1_0142, cya, pXO1-122
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: C2
Unit cell:
a: 317.509Å b: 183.348Å c: 141.812Å
α: 90° β: 90.05° γ: 90°
R-values:
R R work R free
0.286 0.286 0.307
Expression system: Escherichia coli BL21