› 1y0j

Solution NMR

Zinc fingers as protein recognition motifs: structural basis for the GATA-1/Friend of GATA interaction

Released:
DOI: 10.2210/pdb1y0j/pdb
PDB entry 1y0j coloured by chain, ensemble of 20 models, front view.
PDB entry 1y0j coloured by chain, ensemble of 20 models, side view.
PDB entry 1y0j coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
Zinc fingers as protein recognition motifs: structural basis for the GATA-1/friend of GATA interaction.
Liew CK, Simpson RJ, Kwan AH, Crofts LA, Loughlin FE, Matthews JM, Crossley M, Mackay JP
Proc Natl Acad Sci U S A 102 583-8 (2005)
PMID: 15644435

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148130 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Erythroid transcription factor Chain: A
Molecule details ›
Chain: A
Length: 46 amino acids
Theoretical weight: 5.2 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17679 (Residues: 200-243; Coverage: 11%)
Gene names: Gata1, Gf-1
Sequence domains: GATA zinc finger
Zinc finger protein ush Chain: B
Molecule details ›
Chain: B
Length: 36 amino acids
Theoretical weight: 3.96 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9VPQ6 (Residues: 202-235; Coverage: 3%)
Gene names: CG2762, ush

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing molecular dynamics torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Keep your fingers off my DNA: protein-protein interactions mediated by C2H2 zinc finger domains.
Brayer et al. (2008)
16 more

4 mentions without citation

Zinc fingers as protein recognition motifs: structural basis for the GATA-1/friend of GATA interaction.
Liew et al. (2005)
3 more