PDBe 1xrs

X-ray diffraction
2.8Å resolution

Crystal structure of Lysine 5,6-Aminomutase in complex with PLP, cobalamin, and 5'-deoxyadenosine

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lysine 5,6-aminomutase beta subunit Chain: B
Molecule details ›
Chain: B
Length: 262 amino acids
Theoretical weight: 29.25 KDa
Source organism: Acetoanaerobium sticklandii
Expression system: Escherichia coli
UniProt:
  • Canonical: E3PRJ4 (Residues: 1-262; Coverage: 100%)
Gene names: CLOST_1378, kamE
Sequence domains:
Structure domains:
Lysine 5,6-aminomutase alpha subunit Chain: A
Molecule details ›
Chain: A
Length: 516 amino acids
Theoretical weight: 57.38 KDa
Source organism: Acetoanaerobium sticklandii
Expression system: Escherichia coli
UniProt:
  • Canonical: E3PRJ5 (Residues: 4-519; Coverage: 99%)
Gene names: CLOST_1379, kamD
Sequence domains: D-Lysine 5,6-aminomutase TIM-barrel domain of alpha subunit
Structure domains: D-lysine 5,6-aminomutase alpha subunit. Chain A

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P3121
Unit cell:
a: 99.7Å b: 99.7Å c: 168.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.199 0.262
Expression system: Escherichia coli