› 1xqm

X-ray diffraction
2.1Å resolution

Variations on the GFP chromophore scaffold: A fragmented 5-membered heterocycle revealed in the 2.1A crystal structure of a non-fluorescent chromoprotein

Released:
DOI: 10.2210/pdb1xqm/pdb
PDB entry 1xqm coloured by chain, front view.
PDB entry 1xqm coloured by chain, side view.
PDB entry 1xqm coloured by chain, top view.
Source organism: Anemonia sulcata
Primary publication:
Variations on the GFP chromophore: A polypeptide fragmentation within the chromophore revealed in the 2.1-A crystal structure of a nonfluorescent chromoprotein from Anemonia sulcata.
Wilmann PG, Petersen J, Devenish RJ, Prescott M, Rossjohn J
J Biol Chem 280 2401-4 (2005)
PMID: 15542608

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190439 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GFP-like non-fluorescent chromoprotein FP595 Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 25.84 KDa
Source organism: Anemonia sulcata
Expression system: Not provided
UniProt:
  • Canonical: Q9GZ28 (Residues: 1-232; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand ACY 1 x ACY
1 modified residue:
Ligand CH6 1 x CH6

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P6222
Unit cell:
a: 112.482Å b: 112.482Å c: 96.906Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.214 0.233
Expression system: Not provided

Quick links

Citations

14 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
13 more

3 mentions without citation

Chromophore transformations in red fluorescent proteins.
Subach et al. (2012)
2 more