› 1xpi

X-ray diffraction
2.2Å resolution

Crystal structure of the catalytic domain of E. coli pseudouridine synthase RluC

Released:
DOI: 10.2210/pdb1xpi/pdb
PDB entry 1xpi coloured by chain, front view.
PDB entry 1xpi coloured by chain, side view.
PDB entry 1xpi coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Del Campo M, Ofengand J, Fenna R, Malhotra A

Function and Biology Details

Reaction catalysed: 
23S rRNA uridine(955)/uridine(2504)/uridine(2580) = 23S rRNA pseudouridine(955)/pseudouridine(2504)/pseudouridine(2580)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-141970 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal large subunit pseudouridine synthase C Chains: A, B
Molecule details ›
Chains: A, B
Length: 231 amino acids
Theoretical weight: 26.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AA39 (Residues: 89-319; Coverage: 72%)
Gene names: JW1072, b1086, rluC, yceC
Sequence domains: RNA pseudouridylate synthase
Structure domains: Pseudouridine synthase

Ligands and Environments

1 bound ligand:
Ligand ACY 5 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P3221
Unit cell:
a: 97.307Å b: 97.307Å c: 86.237Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.185 0.254
Expression system: Escherichia coli BL21(DE3)

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