› 1xo1

X-ray diffraction
2.5Å resolution

T5 5'-EXONUCLEASE MUTANT K83A

Released:
DOI: 10.2210/pdb1xo1/pdb
PDB entry 1xo1 coloured by chain, front view.
PDB entry 1xo1 coloured by chain, side view.
PDB entry 1xo1 coloured by chain, top view.
Source organism: Escherichia virus T5
Primary publication:
Mutagenesis of conserved lysine residues in bacteriophage T5 5'-3' exonuclease suggests separate mechanisms of endo-and exonucleolytic cleavage.
Garforth SJ, Ceska TA, Suck D, Sayers JR
Proc Natl Acad Sci U S A 96 38-43 (1999)
PMID: 9874768

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138947 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flap endonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 291 amino acids
Theoretical weight: 33.43 KDa
Source organism: Escherichia virus T5
Expression system: Escherichia coli
UniProt:
  • Canonical: P06229 (Residues: 1-291; Coverage: 100%)
Gene names: D15, T5.130, T5p128, exo5
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 66.89Å b: 76.81Å c: 120.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.309
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Single-strand-specific nucleases.
Desai et al. (2003)
18 other articles

4 mentions without citation

Prediction of functional sites by analysis of sequence and structure conservation.
Panchenko et al. (2004)
3 more