PDBe 1xls

X-ray diffraction
2.96Å resolution

Crystal structure of the mouse CAR/RXR LBD heterodimer bound to TCPOBOP and 9cRA and a TIF2 peptide containg the third LXXLL motifs

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero 16-mer
hetero octamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Nuclear receptor subfamily 1 group I member 3 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 242 amino acids
Theoretical weight: 28 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O35627 (Residues: 117-358; Coverage: 68%)
Gene names: Car, Nr1i3
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 2 Chains: I, J, K, L, M, N, O, P
Molecule details ›
Chains: I, J, K, L, M, N, O, P
Length: 18 amino acids
Theoretical weight: 2.13 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: Q9WUI9 (Residues: 739-756; Coverage: 1%)
Gene names: Ncoa2, Tif2
Retinoic acid receptor RXR-alpha Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 232 amino acids
Theoretical weight: 26.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19793 (Residues: 227-458; Coverage: 50%)
Gene names: NR2B1, RXRA
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: P1
Unit cell:
a: 68.271Å b: 88.381Å c: 105.494Å
α: 79.02° β: 85.81° γ: 67.22°
R-values:
R R work R free
0.259 0.255 0.303
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided