PDBe 1xhu

X-ray diffraction
2.95Å resolution

HincII bound to cleaved, cognate DNA containing GTCGAC

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Type-2 restriction enzyme HincII Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 257 amino acids
Theoretical weight: 29.82 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P17743 (Residues: 2-258; Coverage: 100%)
Gene name: hincIIR
Sequence domains: Restriction endonuclease HincII
Structure domains: ECO RV Endonuclease; Chain A
5'-D(P*GP*AP*CP*CP*GP*G)-3' Chains: F, H, J, L
Molecule details ›
Chains: F, H, J, L
Length: 6 nucleotides
Theoretical weight: 1.83 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*GP*CP*CP*GP*GP*TP*C)-3' Chains: E, G, I, K
Molecule details ›
Chains: E, G, I, K
Length: 7 nucleotides
Theoretical weight: 2.11 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: I212121
Unit cell:
a: 67.2Å b: 177.2Å c: 256Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.277
Expression systems:
  • Escherichia coli
  • Not provided