PDBe 1xhm

X-ray diffraction
2.7Å resolution

The Crystal Structure of a Biologically Active Peptide (SIGK) Bound to a G Protein Beta:Gamma Heterodimer

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
SIGK Peptide Chain: C
Molecule details ›
Chain: C
Length: 15 amino acids
Theoretical weight: 1.69 KDa
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62871 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: B
Molecule details ›
Chain: B
Length: 77 amino acids
Theoretical weight: 8.69 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Structure domains: Transducin (heterotrimeric G protein), gamma chain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 45.468Å b: 74.669Å c: 108.023Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.262 0.227 0.287
Expression system: Spodoptera frugiperda