PDBe 1xfw

X-ray diffraction
3.4Å resolution

Crystal structure of anthrax edema factor (EF) in complex with calmodulin and 3'5' cyclic AMP (cAMP)

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chains: O, P, Q, R, S, T
Molecule details ›
Chains: O, P, Q, R, S, T
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Structure domains: EF-hand
Calmodulin-sensitive adenylate cyclase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 777 amino acids
Theoretical weight: 90.13 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
UniProt:
  • Canonical: P40136 (Residues: 33-800; Coverage: 100%)
Gene names: BXA0141, GBAA_pXO1_0142, cya, pXO1-122
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: C2
Unit cell:
a: 320.501Å b: 185.044Å c: 142.454Å
α: 90° β: 90.22° γ: 90°
R-values:
R R work R free
0.266 0.266 0.283
Expression system: Escherichia coli