PDBe 1xfu

X-ray diffraction
3.35Å resolution

Crystal structure of anthrax edema factor (EF) truncation mutant, EF-delta 64 in complex with calmodulin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-2 Chains: O, P, Q, R, S, T
Molecule details ›
Chains: O, P, Q, R, S, T
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0DP24 (Residues: 1-149; Coverage: 100%)
Gene names: CALM2, CAM2, CAMB
Structure domains: EF-hand
Calmodulin-sensitive adenylate cyclase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 747 amino acids
Theoretical weight: 86.43 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P40136 (Residues: 64-800; Coverage: 96%)
Gene names: BXA0141, GBAA_pXO1_0142, cya, pXO1-122
Sequence domains: Anthrax toxin LF subunit
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 317.562Å b: 183.108Å c: 141.117Å
α: 90° β: 90.05° γ: 90°
R-values:
R R work R free
0.283 0.283 0.3
Expression system: Escherichia coli BL21