› 1xfq

Solution NMR

structure of the blue shifted intermediate state of the photoactive yellow protein lacking the N-terminal part

Released:
DOI: 10.2210/pdb1xfq/pdb
PDB entry 1xfq coloured by chain, ensemble of 20 models, front view.
PDB entry 1xfq coloured by chain, ensemble of 20 models, side view.
PDB entry 1xfq coloured by chain, ensemble of 20 models, top view.
Source organism: Halorhodospira halophila
Primary publication:
The solution structure of a transient photoreceptor intermediate: Delta25 photoactive yellow protein.
Bernard C, Houben K, Derix NM, Marks D, van der Horst MA, Hellingwerf KJ, Boelens R, Kaptein R, van Nuland NA
Structure 13 953-62 (2005)
PMID: 16004868

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Photoactive yellow protein Chain: A
Molecule details ›
Chain: A
Length: 113 amino acids
Theoretical weight: 12.82 KDa
Source organism: Halorhodospira halophila
Expression system: Escherichia coli
UniProt:
  • Canonical: P16113 (Residues: 26-125; Coverage: 80%)
Gene name: pyp
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand HC4 1 x HC4
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics, simulated annealing
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Using NMR chemical shifts to calculate the propensity for structural order and disorder in proteins.
Tamiola et al. (2012)
5 more

5 mentions without citation

Improving a designed photocontrolled DNA-binding protein.
Fan et al. (2011)
4 more