› 1xet

X-ray diffraction
2Å resolution

Crystal structure of stilbene synthase from Pinus sylvestris, complexed with methylmalonyl CoA

Released:
DOI: 10.2210/pdb1xet/pdb
PDB entry 1xet coloured by chain, front view.
PDB entry 1xet coloured by chain, side view.
PDB entry 1xet coloured by chain, top view.
Source organism: Pinus sylvestris
Entry authors: Ng SH, Chirgadze D, Spiteller D, Li TL, Spencer JB, Blundell TL

Function and Biology Details

Reaction catalysed: 
3 malonyl-CoA + cinnamoyl-CoA = 4 CoA + pinosylvin + 4 CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169709 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pinosylvin synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 413 amino acids
Theoretical weight: 44.95 KDa
Source organism: Pinus sylvestris
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02323 (Residues: 1-393; Coverage: 100%)
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments


Cofactor: Ligand MCA 1 x MCA
1 bound ligand:
Ligand 3IO 3 x 3IO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21
Unit cell:
a: 54.465Å b: 111.19Å c: 132.015Å
α: 90° β: 93.13° γ: 90°
R-values:
R R work R free
0.195 0.195 0.223
Expression system: Escherichia coli BL21(DE3)

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