PDBe 1xdk

X-ray diffraction
2.9Å resolution

Crystal Structure of the RARbeta/RXRalpha Ligand Binding Domain Heterodimer in Complex with 9-cis Retinoic Acid and a Fragment of the TRAP220 Coactivator

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Retinoic acid receptor beta Chains: B, F
Molecule details ›
Chains: B, F
Length: 303 amino acids
Theoretical weight: 33.98 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22605 (Residues: 180-482; Coverage: 63%)
Gene names: Nr1b2, Rarb
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Retinoic acid receptor RXR-alpha Chains: A, E
Molecule details ›
Chains: A, E
Length: 238 amino acids
Theoretical weight: 26.58 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28700 (Residues: 230-467; Coverage: 51%)
Gene names: Nr2b1, Rxra
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Mediator of RNA polymerase II transcription subunit 1 Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 14 amino acids
Theoretical weight: 1.61 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q925J9 (Residues: 641-654; Coverage: 1%)
Gene names: Crsp210, Drip205, Med1, Pbp, Pparbp, Trap220, Trip2

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P3121
Unit cell:
a: 115.7Å b: 115.7Å c: 247.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.253 0.253 0.296
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided