1x6b Experiments and Validation

Solution NMR

Source organism: Homo sapiens [9606]
Refinement method: torsion angle dynamics
Number of deposited models: 20 models
Chemical shifts: BMR10241  

Sample information

Author description:
Solution structures of the SH3 domain of human rho guanine exchange factor (GEF) 16
Source organism: Homo sapiens [9606]
Expression system: Not provided

Validation information

 
Structure ensemble information
Refinement method: torsion angle dynamics
Number of calculated models: 100
Number of deposited models: 20 (target function,structures with the least restraint violations)
Representative model: 1 (lowest energy)
 
Experimental NMR data
Chemical shifts: BMR10241  

Experimental information

Sample 1
Contents: 1mM SH3 domain U-15N, 13C; 20mM d-Tris HCl; 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 10% D2O Recorded spectra: 3D_13C-separated_NOESY, 3D_15N-separated_NOESY

Samples and spectra
NMR spectrometers used
Bruker AVANCE 800 MHz