› 1wzi

X-ray diffraction
2Å resolution

Structural basis for alteration of cofactor specificity of Malate dehydrogenase from Thermus flavus

Released:
DOI: 10.2210/pdb1wzi/pdb
PDB entry 1wzi coloured by chain, front view.
PDB entry 1wzi coloured by chain, side view.
PDB entry 1wzi coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Tomita T, Fushinobu S, Kuzuyama T, Nishiyama M

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 327 amino acids
Theoretical weight: 35.46 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10584 (Residues: 1-327; Coverage: 100%)
Gene name: mdh
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NDP 2 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 70.31Å b: 85.39Å c: 117.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.229
Expression system: Escherichia coli

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