PDBe 1wxh

X-ray diffraction
1.9Å resolution

E.coli NAD Synthetase, NAD

Released:

Function and Biology Details

Reaction catalysed:
ATP + deamido-NAD(+) + NH(3) = AMP + diphosphate + NAD(+)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NH(3)-dependent NAD(+) synthetase Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 30.67 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18843 (Residues: 1-275; Coverage: 100%)
Gene names: JW1729, b1740, efg, nadE, ntrL
Sequence domains: NAD synthase
Structure domains: HUPs

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 92.239Å b: 92.239Å c: 76.239Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.171 0.191
Expression system: Escherichia coli BL21(DE3)