PDBe 1wtp

X-ray diffraction
1.9Å resolution

Hyperthermophile chromosomal protein SAC7D single mutant M29F in complex with DNA GCGA(UBr)CGC

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA-binding protein 7d Chains: A, B
Molecule details ›
Chains: A, B
Length: 66 amino acids
Theoretical weight: 7.64 KDa
Source organism: Sulfolobus acidocaldarius
Expression system: Escherichia coli
UniProt:
  • Canonical: P13123 (Residues: 1-66; Coverage: 100%)
Gene name: Saci_0064
Sequence domains: 7kD DNA-binding domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
5'-D(*GP*CP*GP*AP*(BRU)P*CP*GP*C)-3' Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 8 nucleotides
Theoretical weight: 2.49 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-002
Spacegroup: P21
Unit cell:
a: 38.217Å b: 47.889Å c: 52.494Å
α: 90° β: 102.67° γ: 90°
R-values:
R R work R free
0.228 0.222 0.287
Expression systems:
  • Escherichia coli
  • Not provided