› 1wrv

X-ray diffraction
1.5Å resolution

Crystal Structure of T.th.HB8 Branched-Chain Amino Acid Aminotransferase

Released:
DOI: 10.2210/pdb1wrv/pdb
PDB entry 1wrv coloured by chain, front view.
PDB entry 1wrv coloured by chain, side view.
PDB entry 1wrv coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Goto M, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer
homo hexamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178234 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Branched-chain-amino-acid aminotransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 308 amino acids
Theoretical weight: 34.07 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SM19 (Residues: 1-308; Coverage: 100%)
Gene names: TTHA0124, ilvE
Sequence domains: Amino-transferase class IV
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 3 x PLP
2 bound ligands:
Ligand CL 3 x CL
Ligand MPD 5 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45PX
Spacegroup: P41212
Unit cell:
a: 143.41Å b: 143.41Å c: 116.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.218
Expression system: Escherichia coli BL21(DE3)

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