PDBe 1wcz

X-ray diffraction
2Å resolution

Crystal structure of an alkaline form of v8 protease from Staphylococcus aureus

Released:
Source organism: Staphylococcus aureus
Entry authors: Yamada K, Ohta M, Hasegawa T, Torii K, Murakami M, Kouyama K

Function and Biology Details

Reaction catalysed:
Preferential cleavage: Glu-|-Xaa, Asp-|-Xaa. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamyl endopeptidase Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 29.02 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: P0C1U8 (Residues: 69-336; Coverage: 87%)
Gene name: sspA
Sequence domains: Trypsin-like peptidase domain
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: P6522
Unit cell:
a: 61.29Å b: 61.29Å c: 221.13Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.205 0.221