› 1w47

X-ray diffraction
2.5Å resolution

P4 protein from Bacteriophage PHI12 in complex with ADP and MN

Released:
DOI: 10.2210/pdb1w47/pdb
PDB entry 1w47 coloured by chain, front view.
PDB entry 1w47 coloured by chain, side view.
PDB entry 1w47 coloured by chain, top view.
Source organism: Pseudomonas phage phi12
Primary publication:
Atomic snapshots of an RNA packaging motor reveal conformational changes linking ATP hydrolysis to RNA translocation.
Mancini EJ, Kainov DE, Grimes JM, Tuma R, Bamford DH, Stuart DI
Cell 118 743-55 (2004)
PMID: 15369673

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188245 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NTPase P4 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 331 amino acids
Theoretical weight: 35.15 KDa
Source organism: Pseudomonas phage phi12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q94M05 (Residues: 1-331; Coverage: 100%)
Gene name: 4
Sequence domains: ATPase P4 of dsRNA bacteriophage phi-12
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 3 x ADP
Ligand MN 3 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: I222
Unit cell:
a: 104.8Å b: 128.2Å c: 158.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 0.198
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Molecular chaperones and protein quality control.
Bukau et al. (2006)
26 more