Function and Biology

Native Cytokinin Dehydrogenase

Source organism: Zea mays
Biochemical function: cytokinin dehydrogenase activity
Biological process: cytokinin metabolic process
Cellular component: not assigned

EC 1.5.99.12: Cytokinin dehydrogenase

Reaction catalysed:
N(6)-dimethylallyladenine + acceptor + H(2)O = adenine + 3-methylbut-2- enal + reduced acceptor.
Comments:
  • Converts zeatin and related cytokinins.
  • Catalyzes the oxidation of cytokinins, a family of N(6)-substituted adenine derivatives that are plant hormones, where the substituent is a dimethylallyl or other prenyl group.
  • Although this activity was previously thought to be catalyzed by a hydrogen-peroxide-forming oxidase, this enzyme does not require oxygen for activity and does not form hydrogen peroxide.
  • 2,6-dichloroindophenol, methylene blue, nitroblue tetrazolium, phenazine methosulfate and Cu(2+) in the presence of imidazole can act as acceptors.
Systematic name:
N(6)-dimethylallyladenine:acceptor oxidoreductase
Alternative Name(s):
  • Cytokinin oxidase
  • N(6)-dimethylallyladenine:(acceptor) oxidoreductase
  • 6-N-dimethylallyladenine:acceptor oxidoreductase

Sequence families

Pfam Protein families (Pfam)
PF01565
Domain description: FAD binding domain
Occurring in:
  1. Cytokinin dehydrogenase 1
1 copy of Pfam domain PF01565 (FAD binding domain) in Cytokinin dehydrogenase 1 in PDB 1w1o.

PF09265
Domain description: Cytokinin dehydrogenase 1, FAD and cytokinin binding
Occurring in:
  1. Cytokinin dehydrogenase 1
1 copy of Pfam domain PF09265 (Cytokinin dehydrogenase 1, FAD and cytokinin binding) in Cytokinin dehydrogenase 1 in PDB 1w1o.

InterPro InterPro annotations
IPR036318
Domain description: FAD-binding, type 2-like superfamily
Occurring in:
  1. Cytokinin dehydrogenase 1
IPR016170
Domain description: Vanillyl-alcohol oxidase/Cytokinin dehydrogenase C-terminal domain superfamily
Occurring in:
  1. Cytokinin dehydrogenase 1
IPR016164
Domain description: FAD-linked oxidase-like, C-terminal
Occurring in:
  1. Cytokinin dehydrogenase 1
IPR006094
Domain description: FAD linked oxidase, N-terminal
Occurring in:
  1. Cytokinin dehydrogenase 1
IPR016166
Domain description: FAD-binding, type 2
Occurring in:
  1. Cytokinin dehydrogenase 1
IPR016167
Domain description: FAD-binding, type 2, subdomain 1
Occurring in:
  1. Cytokinin dehydrogenase 1
IPR006093
Domain description: Oxygen oxidoreductase covalent FAD-binding site
Occurring in:
  1. Cytokinin dehydrogenase 1
IPR016169
Domain description: CO dehydrogenase flavoprotein-like, FAD-binding, subdomain 2
Occurring in:
  1. Cytokinin dehydrogenase 1
IPR015345
Domain description: Cytokinin dehydrogenase 1, FAD/cytokinin binding domain
Occurring in:
  1. Cytokinin dehydrogenase 1

Structure domains

CATH CATH domains
3.40.462.10
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Vanillyl-alcohol Oxidase; Chain A, domain 3
Homology: FAD-linked oxidases, C-terminal domain
Occurring in:
  1. Cytokinin dehydrogenase 1
1 copy of CATH domain 3.40.462.10 (Vanillyl-alcohol Oxidase; Chain A, domain 3) in Cytokinin dehydrogenase 1 in PDB 1w1o.
3.30.465.10
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 3
Homology: Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 3
Occurring in:
  1. Cytokinin dehydrogenase 1
1 copy of CATH domain 3.30.465.10 (Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 3) in Cytokinin dehydrogenase 1 in PDB 1w1o.
SCOP SCOP annotations
56177
Class: Alpha and beta proteins (a+b)
Fold: FAD-binding/transporter-associated domain-like
Superfamily: FAD-binding/transporter-associated domain-like
Occurring in:
  1. Cytokinin dehydrogenase 1
1 copy of SCOP domain 56177 (FAD-linked oxidases, N-terminal domain) in Cytokinin dehydrogenase 1 in PDB 1w1o.
110990
Class: Alpha and beta proteins (a+b)
Fold: Ferredoxin-like
Superfamily: FAD-linked oxidases, C-terminal domain
Occurring in:
  1. Cytokinin dehydrogenase 1
1 copy of SCOP domain 110990 (Cytokinin dehydrogenase 1) in Cytokinin dehydrogenase 1 in PDB 1w1o.