PDBe 1vzx

X-ray diffraction
1.97Å resolution

Roles of active site tryptophans in substrate binding and catalysis by ALPHA-1,3 GALACTOSYLTRANSFERASE

Released:

Function and Biology Details

Reaction catalysed:
UDP-alpha-D-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D- glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl- (1->4)-beta-N-acetylglucosaminyl-R. 
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyllactosaminide alpha-1,3-galactosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 34.07 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P14769 (Residues: 80-368; Coverage: 79%)
Gene name: GGTA1
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: P21
Unit cell:
a: 45.22Å b: 94.48Å c: 94.447Å
α: 90° β: 99° γ: 90°
R-values:
R R work R free
0.154 0.154 0.202
Expression system: Escherichia coli