PDBe 1vyh

X-ray diffraction
3.4Å resolution

PAF-AH Holoenzyme: Lis1/Alfa2

Released:

Function and Biology Details

Reaction catalysed:
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H(2)O = 1-alkyl-sn-glycero-3-phosphocholine + acetate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Platelet-activating factor acetylhydrolase IB subunit beta Chains: A, B, E, F, I, J, M, N, Q, R
Molecule details ›
Chains: A, B, E, F, I, J, M, N, Q, R
Length: 229 amino acids
Theoretical weight: 25.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P68402 (Residues: 1-229; Coverage: 100%)
Gene names: PAFAH1B2, PAFAHB
Sequence domains: GDSL-like Lipase/Acylhydrolase family
Structure domains: SGNH hydrolase
Platelet-activating factor acetylhydrolase IB subunit alpha Chains: C, D, G, H, K, L, O, P, S, T
Molecule details ›
Chains: C, D, G, H, K, L, O, P, S, T
Length: 410 amino acids
Theoretical weight: 46.79 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63005 (Residues: 1-410; Coverage: 100%)
Gene names: Lis-1, Lis1, Pafah1b1, Pafaha
Sequence domains:
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 205.77Å b: 101.22Å c: 246.13Å
α: 90° β: 98.06° γ: 90°
R-values:
R R work R free
0.265 0.264 0.307
Expression systems:
  • Escherichia coli BL21(DE3)
  • Spodoptera frugiperda