PDBe 1vra

X-ray diffraction
2Å resolution

Crystal structure of Arginine biosynthesis bifunctional protein argJ (10175521) from Bacillus halodurans at 2.00 A resolution

Released:
Source organism: Bacillus halodurans
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reactions catalysed:
Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate
N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Arginine biosynthesis bifunctional protein ArgJ alpha chain Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 22.35 KDa
Source organism: Bacillus halodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW Q9K8V3 (Residues: 1-196; Coverage: 48%)
Gene names: BH2899, argJ
Structure domains: L-amino peptidase D-ALA esterase/amidase
Arginine biosynthesis bifunctional protein ArgJ beta chain Chain: B
Molecule details ›
Chain: B
Length: 215 amino acids
Theoretical weight: 23.2 KDa
Source organism: Bacillus halodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW Q9K8V3 (Residues: 197-411; Coverage: 52%)
Gene names: BH2899, argJ
Structure domains:

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P41212
Unit cell:
a: 70.658Å b: 70.658Å c: 222.292Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 0.14 0.158
Expression system: Escherichia coli