PDBe 1vff

X-ray diffraction
2.5Å resolution

beta-glycosidase from Pyrococcus horikoshii

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
423aa long hypothetical beta-glucosidase Chain: A
Molecule details ›
Chain: A
Length: 423 amino acids
Theoretical weight: 50.4 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O58104 (Residues: 1-423; Coverage: 100%)
Gene name: PH0366
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: C2
Unit cell:
a: 122.56Å b: 61.17Å c: 73.74Å
α: 90° β: 92.47° γ: 90°
R-values:
R R work R free
0.176 0.176 0.231
Expression system: Escherichia coli BL21(DE3)