Function and Biology

STRUCTURE OF THE OCTAMERIC FLAVOENZYME VANILLYL-ALCOHOL OXIDASE

Source organism: Penicillium simplicissimum
Biochemical function: catalytic activity
Biological process: methanol metabolic process
Cellular component: peroxisome

EC 1.1.3.38: Vanillyl-alcohol oxidase

Reaction catalysed:
Vanillyl alcohol + O(2) = vanillin + H(2)O(2).
Comments:
  • Converts a wide range of 4-hydroxybenzyl alcohols and 4-hydroxybenzylamines into the corresponding aldehydes.
  • The allyl group of 4-allylphenols is also converted into the -CH=CH-CH(2)OH group.
Systematic name:
Vanillyl alcohol:oxygen oxidoreductase
Alternative Name(s):
  • 4-hydroxy-2-methoxybenzyl alcohol oxidase

Sequence families

Pfam Protein families (Pfam)
PF02913
Domain description: FAD linked oxidases, C-terminal domain
Occurring in:
  1. Vanillyl-alcohol oxidase
1 copy of Pfam domain PF02913 (FAD linked oxidases, C-terminal domain) in Vanillyl-alcohol oxidase in PDB 1vao.

PF01565
Domain description: FAD binding domain
Occurring in:
  1. Vanillyl-alcohol oxidase
1 copy of Pfam domain PF01565 (FAD binding domain) in Vanillyl-alcohol oxidase in PDB 1vao.

InterPro InterPro annotations
IPR036318
Domain description: FAD-binding, type 2-like superfamily
Occurring in:
  1. Vanillyl-alcohol oxidase
IPR016170
Domain description: Cytokinin dehydrogenase, C-terminal domain superfamily
Occurring in:
  1. Vanillyl-alcohol oxidase
IPR016171
Domain description: Vanillyl-alcohol oxidase, C-terminal subdomain 2
Occurring in:
  1. Vanillyl-alcohol oxidase
IPR016164
Domain description: FAD-linked oxidase-like, C-terminal
Occurring in:
  1. Vanillyl-alcohol oxidase
IPR006094
Domain description: FAD linked oxidase, N-terminal
Occurring in:
  1. Vanillyl-alcohol oxidase
IPR004113
Domain description: FAD-linked oxidase, C-terminal
Occurring in:
  1. Vanillyl-alcohol oxidase
IPR016166
Domain description: FAD-binding, type 2
Occurring in:
  1. Vanillyl-alcohol oxidase
IPR016167
Domain description: FAD-binding, type 2, subdomain 1
Occurring in:
  1. Vanillyl-alcohol oxidase
IPR016169
Domain description: CO dehydrogenase flavoprotein-like, FAD-binding, subdomain 2
Occurring in:
  1. Vanillyl-alcohol oxidase

Structure domains

CATH CATH domains
3.30.465.10
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 3
Homology: Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 3
Occurring in:
  1. Vanillyl-alcohol oxidase
1 copy of CATH domain 3.30.465.10 (Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 3) in Vanillyl-alcohol oxidase in PDB 1vao.
3.30.43.10
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 2
Homology: Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2
Occurring in:
  1. Vanillyl-alcohol oxidase
1 copy of CATH domain 3.30.43.10 (Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 2) in Vanillyl-alcohol oxidase in PDB 1vao.
1.10.45.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Vanillyl-alcohol Oxidase; Chain A, domain 4
Homology: Vanillyl-alcohol Oxidase; Chain A, domain 4
Occurring in:
  1. Vanillyl-alcohol oxidase
1 copy of CATH domain 1.10.45.10 (Vanillyl-alcohol Oxidase; Chain A, domain 4) in Vanillyl-alcohol oxidase in PDB 1vao.
3.40.462.10
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Vanillyl-alcohol Oxidase; Chain A, domain 3
Homology: FAD-linked oxidases, C-terminal domain
Occurring in:
  1. Vanillyl-alcohol oxidase
1 copy of CATH domain 3.40.462.10 (Vanillyl-alcohol Oxidase; Chain A, domain 3) in Vanillyl-alcohol oxidase in PDB 1vao.
SCOP SCOP annotations
55104
Class: Alpha and beta proteins (a+b)
Fold: Ferredoxin-like
Superfamily: FAD-linked oxidases, C-terminal domain
Occurring in:
  1. Vanillyl-alcohol oxidase
1 copy of SCOP domain 55104 (Vanillyl-alcohol oxidase-like) in Vanillyl-alcohol oxidase in PDB 1vao.
56177
Class: Alpha and beta proteins (a+b)
Fold: FAD-binding/transporter-associated domain-like
Superfamily: FAD-binding/transporter-associated domain-like
Occurring in:
  1. Vanillyl-alcohol oxidase
1 copy of SCOP domain 56177 (FAD-linked oxidases, N-terminal domain) in Vanillyl-alcohol oxidase in PDB 1vao.