PDBe 1v08

X-ray diffraction
1.9Å resolution

Function and Biology Details

Reactions catalysed:
(1) (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H(2)O = 2,4-dihydroxy-7-methoxy-2H-1,4- benzoxazin-3(4H)-one + D-glucose. (2) (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D- glucopyranoside + H(2)O = 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose. 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 512 amino acids
Theoretical weight: 58.46 KDa
Source organism: Zea mays
Expression system: Escherichia coli
UniProt:
  • Canonical: P49235 (Residues: 55-566; Coverage: 91%)
Gene name: GLU1
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 96.18Å b: 104.8Å c: 119.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.205
Expression system: Escherichia coli