› 1uzr

X-ray diffraction
2.2Å resolution

Crystal Structure of the Class Ib Ribonucleotide Reductase R2F-2 subunit from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb1uzr/pdb
PDB entry 1uzr coloured by chain, front view.
PDB entry 1uzr coloured by chain, side view.
PDB entry 1uzr coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of the biologically active form of class Ib ribonucleotide reductase small subunit from Mycobacterium tuberculosis.
Uppsten M, Davis J, Rubin H, Uhlin U
FEBS Lett 569 117-22 (2004)
PMID: 15225619

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161543 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase subunit beta nrdF2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 296 amino acids
Theoretical weight: 33.84 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WH71 (Residues: 1-296; Coverage: 91%)
Gene names: Rv3048c, nrdF2
Sequence domains: Ribonucleotide reductase, small chain
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

3 bound ligands:
Ligand FE 6 x FE
Ligand CIT 3 x CIT
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P42212
Unit cell:
a: 161.492Å b: 161.492Å c: 115.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.202
Expression system: Escherichia coli

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Citations

5 review citations

Recent advancements in the development of anti-tuberculosis drugs.
Chetty et al. (2017)
4 more

4 mentions without citation

Defining the Domain Arrangement of the Mammalian Target of Rapamycin Complex Component Rictor Protein.
Zhou et al. (2015)
3 more