PDBe 1uz4

X-ray diffraction
1.71Å resolution

Common inhibition of beta-glucosidase and beta-mannosidase by isofagomine lactam reflects different conformational intineraries for glucoside and mannoside hydrolysis


Function and Biology Details

Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Man5A Chain: A
Molecule details ›
Chain: A
Length: 440 amino acids
Theoretical weight: 50.23 KDa
Source organism: Cellvibrio mixtus
Expression system: Escherichia coli BL21
  • Canonical: Q6QT42 (Residues: 26-456; Coverage: 95%)
Gene name: man5A
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21212
Unit cell:
a: 91.625Å b: 102.37Å c: 50.356Å
α: 90° β: 90° γ: 90°
R R work R free
0.135 0.133 0.165
Expression system: Escherichia coli BL21