› 1uz2

X-ray diffraction
1.95Å resolution

The Cys121Ser Mutant of Beta-Lactoglobulin

Released:
DOI: 10.2210/pdb1uz2/pdb
PDB entry 1uz2 coloured by chain, front view.
PDB entry 1uz2 coloured by chain, side view.
PDB entry 1uz2 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
A recombinant C121S mutant of bovine beta-lactoglobulin is more susceptible to peptic digestion and to denaturation by reducing agents and heating.
Jayat D, Gaudin JC, Chobert JM, Burova TV, Holt C, McNae I, Sawyer L, Haertlé T
Biochemistry 43 6312-21 (2004)
PMID: 15147215

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactoglobulin Chain: X
Molecule details ›
Chain: X
Length: 162 amino acids
Theoretical weight: 18.37 KDa
Source organism: Bos taurus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P02754 (Residues: 17-178; Coverage: 100%)
Gene name: LGB
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: C2221
Unit cell:
a: 55.69Å b: 80.47Å c: 66.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.267 0.267
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

Disulphide bond formation in food protein aggregation and gelation.
Visschers et al. (2005)
1 more

2 mentions without citation

Relevant B cell epitopes in allergic disease.
Pomés A. (2010)
1 more