PDBe 1uy4

X-ray diffraction
1.69Å resolution

Binding sub-site dissection of a family 6 carbohydrate-binding module by X-ray crystallography and isothermal titration calorimetry

Released:

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase A Chain: A
Molecule details ›
Chain: A
Length: 145 amino acids
Theoretical weight: 15.24 KDa
Source organism: [Clostridium] stercorarium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GJ44 (Residues: 233-373; Coverage: 23%)
Gene name: xynA
Sequence domains: Carbohydrate binding module (family 6)
Structure domains: Galactose-binding domain-like

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-002
Spacegroup: P41212
Unit cell:
a: 83.437Å b: 83.437Å c: 44.921Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.132 0.177
Expression system: Escherichia coli BL21(DE3)