PDBe 1uwr

X-ray diffraction
2.14Å resolution

Structure of beta-glycosidase from Sulfolobus solfataricus in complex with 2-deoxy-2-fluoro-galactose

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D- galactosides. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 489 amino acids
Theoretical weight: 56.76 KDa
Source organism: Sulfolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22498 (Residues: 1-489; Coverage: 100%)
Gene names: SSO3019, lacS
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3121
Unit cell:
a: 168.059Å b: 168.059Å c: 95.773Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.19 0.231
Expression system: Escherichia coli BL21(DE3)