PDBe 1uw9

X-ray diffraction
2.05Å resolution

L290F-A222T chlamydomonas Rubisco mutant

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 16-mer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribulose bisphosphate carboxylase large chain Chains: A, B, E, H, K, O, R, V
Molecule details ›
Chains: A, B, E, H, K, O, R, V
Length: 475 amino acids
Theoretical weight: 52.76 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P00877 (Residues: 1-475; Coverage: 100%)
Gene name: rbcL
Sequence domains:
Structure domains:
Ribulose bisphosphate carboxylase small chain 1, chloroplastic Chains: C, F, I, J, M, P, T, W
Molecule details ›
Chains: C, F, I, J, M, P, T, W
Length: 140 amino acids
Theoretical weight: 16.29 KDa
Source organism: Chlamydomonas reinhardtii
UniProt:
  • Canonical: P00873 (Residues: 46-185; Coverage: 76%)
Gene name: RBCS-1
Sequence domains: Ribulose bisphosphate carboxylase, small chain
Structure domains: RuBisCO, small subunit

Ligands and Environments

3 bound ligands:

3 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 126.073Å b: 178.196Å c: 120.463Å
α: 90° β: 120.35° γ: 90°
R-values:
R R work R free
0.157 0.157 0.194