PDBe 1ush

X-ray diffraction
1.73Å resolution

5'-NUCLEOTIDASE FROM E. COLI

Released:

Function and Biology Details

Reactions catalysed:
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate. 
UDP-sugar + H(2)O = UMP + alpha-D-aldose 1-phosphate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein UshA Chain: A
Molecule details ›
Chain: A
Length: 550 amino acids
Theoretical weight: 60.89 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P07024 (Residues: 1-550; Coverage: 100%)
Gene names: JW0469, b0480, ushA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P41212
Unit cell:
a: 83.61Å b: 83.61Å c: 181.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.219
Expression system: Escherichia coli