PDBe 1ur0

X-ray diffraction
2.5Å resolution

The structure of endo-beta-1,4-galactanase from Bacillus licheniformis in complex with two oligosaccharide products.

Released:

Function and Biology Details

Reaction catalysed:
The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arabinogalactan endo-beta-1,4-galactanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 399 amino acids
Theoretical weight: 43.78 KDa
Source organism: Bacillus licheniformis
Expression system: Bacillus subtilis
UniProt:
  • Canonical: Q65CX5 (Residues: 26-424; Coverage: 100%)
Gene names: BL00263, BLi04276, galA, ganB, yvfO
Sequence domains: Glycosyl hydrolase family 53
Structure domains: Glycosidases

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P21
Unit cell:
a: 50.57Å b: 80.14Å c: 103.22Å
α: 90° β: 99.39° γ: 90°
R-values:
R R work R free
0.204 0.204 0.244
Expression system: Bacillus subtilis