PDBe 1uqt

X-ray diffraction
2Å resolution

Trehalose-6-phosphate from E. coli bound with UDP-2-fluoro glucose.

Released:

Function and Biology Details

Reaction catalysed:
UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate. 

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trehalose-6-phosphate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 482 amino acids
Theoretical weight: 54.75 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P31677 (Residues: 1-474; Coverage: 100%)
Gene names: JW5312, b1896, otsA
Sequence domains: Glycosyltransferase family 20
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF
Spacegroup: P21212
Unit cell:
a: 88.919Å b: 101.521Å c: 118.207Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.219 0.251
Expression system: Escherichia coli BL21