PDBe 1upp

X-ray diffraction
2.3Å resolution

SPINACH RUBISCO IN COMPLEX WITH 2-CARBOXYARABINITOL 2 BISPHOSPHATE and Calcium.

Released:
Source organism: Spinacia oleracea
Primary publication:
Calcium supports loop closure but not catalysis in Rubisco.
J. Mol. Biol. 334 65-73 (2003)
PMID: 14596800

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 16-mer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribulose bisphosphate carboxylase large chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 475 amino acids
Theoretical weight: 52.85 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P00875 (Residues: 1-475; Coverage: 100%)
Gene name: rbcL
Sequence domains:
Structure domains:
Ribulose bisphosphate carboxylase small chain 2, chloroplastic Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 123 amino acids
Theoretical weight: 14.64 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: Q43832 (Residues: 58-180; Coverage: 68%)
Gene name: RBCS2
Sequence domains: Ribulose bisphosphate carboxylase, small chain
Structure domains: RuBisCO, small subunit

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2221
Unit cell:
a: 155.88Å b: 156.25Å c: 199.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 0.256 0.307