PDBe 1upm

X-ray diffraction
2.3Å resolution

ACTIVATED SPINACH RUBISCO COMPLEXED WITH 2-CARBOXYARABINITOL 2 BISPHOSPHAT AND CA2+.

Released:
Source organism: Spinacia oleracea
Primary publication:
Calcium supports loop closure but not catalysis in Rubisco.
J. Mol. Biol. 334 65-73 (2003)
PMID: 14596800

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 16-mer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribulose bisphosphate carboxylase large chain Chains: B, E, H, K, L, O, R, V
Molecule details ›
Chains: B, E, H, K, L, O, R, V
Length: 475 amino acids
Theoretical weight: 52.85 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P00875 (Residues: 1-475; Coverage: 100%)
Gene name: rbcL
Sequence domains:
Structure domains:
Ribulose bisphosphate carboxylase small chain 2, chloroplastic Chains: C, F, I, M, P, S, T, W
Molecule details ›
Chains: C, F, I, M, P, S, T, W
Length: 123 amino acids
Theoretical weight: 14.64 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: Q43832 (Residues: 58-180; Coverage: 68%)
Gene name: RBCS2
Sequence domains: Ribulose bisphosphate carboxylase, small chain
Structure domains: RuBisCO, small subunit

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 219.6Å b: 220.942Å c: 116.984Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.2