PDBe 1unh

X-ray diffraction
2.35Å resolution

Structural mechanism for the inhibition of CDK5-p25 by roscovitine, aloisine and indirubin.

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cyclin-dependent-like kinase 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 292 amino acids
Theoretical weight: 33.35 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q00535 (Residues: 1-292; Coverage: 100%)
Gene names: CDK5, CDKN5
Sequence domains: Protein kinase domain
Structure domains:
Cyclin-dependent kinase 5 activator 1, p25 Chains: D, E
Molecule details ›
Chains: D, E
Length: 208 amino acids
Theoretical weight: 23.2 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q15078 (Residues: 100-307; Coverage: 68%)
Gene names: CDK5R, CDK5R1, NCK5A
Sequence domains: Cyclin-dependent kinase 5 activator protein
Structure domains: Cyclin A; domain 1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 149.541Å b: 90.124Å c: 83.158Å
α: 90° β: 93.29° γ: 90°
R-values:
R R work R free
0.229 0.229 0.23
Expression system: Spodoptera frugiperda