PDBe 1umh

X-ray diffraction
2Å resolution

Structural basis of sugar-recognizing ubiquitin ligase

Released:
Source organism: Mus musculus
Primary publication:
Structural basis of sugar-recognizing ubiquitin ligase.
Nat. Struct. Mol. Biol. 11 365-70 (2004)
PMID: 14990996

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
F-box only protein 2 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 21.02 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q80UW2 (Residues: 117-297; Coverage: 61%)
Gene names: Fbs1, Fbx2, Fbxo2
Sequence domains: F-box associated region
Structure domains: Galactose-binding domain-like

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3221
Unit cell:
a: 62.44Å b: 62.44Å c: 117.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.155 0.153 0.192
Expression system: Escherichia coli BL21(DE3)