› 1uki

X-ray diffraction
2.7Å resolution

Structural basis for the selective inhibition of JNK1 by the scaffolding protein JIP1 and SP600125

Released:
DOI: 10.2210/pdb1uki/pdb
PDB entry 1uki coloured by chain, front view.
PDB entry 1uki coloured by chain, side view.
PDB entry 1uki coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the selective inhibition of JNK1 by the scaffolding protein JIP1 and SP600125.
Heo YS, Kim SK, Seo CI, Kim YK, Sung BJ, Lee HS, Lee JI, Park SY, Kim JH, Hwang KY, Hyun YL, Jeon YH, Ro S, Cho JM, Lee TG, Yang CH
EMBO J 23 2185-95 (2004)
PMID: 15141161

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155387 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitogen-activated protein kinase 8 Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 42.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P45983 (Residues: 1-363; Coverage: 85%)
Gene names: JNK1, MAPK8, PRKM8, SAPK1, SAPK1C
Sequence domains: Protein kinase domain
Structure domains:
C-Jun-amino-terminal kinase-interacting protein 1 Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.35 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q9WVI9 (Residues: 153-163; Coverage: 2%)
Gene names: Ib1, Jip1, Mapk8ip, Mapk8ip1, Prkm8ip

Ligands and Environments

1 bound ligand:
Ligand 537 1 x 537
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6B
Spacegroup: P212121
Unit cell:
a: 61.697Å b: 79.46Å c: 82.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.216 0.241
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

30 review citations

Differential regulation and properties of MAPKs.
Raman et al. (2007)
29 more

40 mentions without citation

Uses for JNK: the many and varied substrates of the c-Jun N-terminal kinases.
Bogoyevitch et al. (2006)
39 more