PDBe 1ukh

X-ray diffraction
2.35Å resolution

Structural basis for the selective inhibition of JNK1 by the scaffolding protein JIP1 and SP600125

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitogen-activated protein kinase 8 Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 42.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P45983 (Residues: 1-363; Coverage: 85%)
  • Best match: P45983-3 (Residues: 1-363)
Gene names: JNK1, MAPK8, PRKM8, SAPK1, SAPK1C
Sequence domains: Protein kinase domain
Structure domains:
C-Jun-amino-terminal kinase-interacting protein 1 Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.35 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q9WVI9 (Residues: 153-163; Coverage: 2%)
Gene names: Ib1, Jip1, Mapk8ip, Mapk8ip1, Prkm8ip

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P212121
Unit cell:
a: 62.398Å b: 80.841Å c: 83.742Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.226 0.245
Expression systems:
  • Escherichia coli
  • Not provided