PDBe 1uk7

X-ray diffraction
1.7Å resolution

Crystal structure of a meta-cleavage product hydrolase (CumD) complexed with n-butyrate

Released:

Function and Biology Details

Reaction catalysed:
2-hydroxymuconate-6-semialdehyde + H(2)O = formate + 2-oxopent-4-enoate. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase Chain: A
Molecule details ›
Chain: A
Length: 282 amino acids
Theoretical weight: 31.51 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P96965 (Residues: 1-282; Coverage: 100%)
Gene name: cumD
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: C2221
Unit cell:
a: 76.91Å b: 116.322Å c: 78.542Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.205
Expression system: Escherichia coli