PDBe 1uj0

X-ray diffraction
1.7Å resolution

Crystal Structure of STAM2 SH3 domain in complex with a UBPY-derived peptide

Released:

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin carboxyl-terminal hydrolase 8 Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.28 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q80U87 (Residues: 699-709; Coverage: 1%)
Gene names: Kiaa0055, Ubpy, Usp8
Signal transducing adapter molecule 2 Chain: A
Molecule details ›
Chain: A
Length: 62 amino acids
Theoretical weight: 7.06 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O88811 (Residues: 204-261; Coverage: 11%)
Gene names: Hbp, Stam2
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P61
Unit cell:
a: 48.454Å b: 48.454Å c: 58.582Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.215 0.23
Expression systems:
  • Not provided
  • Escherichia coli