1ui7

X-ray diffraction
2Å resolution

Site-directed mutagenesis of His433 involved in binding of copper ion in Arthrobacter globiformis amine oxidase

Released:

Function and Biology Details

Reaction catalysed:
RCH(2)NH(2) + H(2)O + O(2) = RCHO + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-155528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylethylamine oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 638 amino acids
Theoretical weight: 70.66 KDa
Source organism: Arthrobacter globiformis
Expression system: Escherichia coli
UniProt:
  • Canonical: P46881 (Residues: 1-638; Coverage: 100%)
Sequence domains: Copper amine oxidase, enzyme domain
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Unit cell:
a: 156.51Å b: 63.32Å c: 185.212Å
α: 90° β: 111.5° γ: 90°
R-values:
R R work R free
0.251 0.206 0.28
Expression system: Escherichia coli