1ug1 Experiments and Validation

Solution NMR

Entry percentile scores
Source organism: Homo sapiens
Refinement method: torsion angle dynamics, simulated annealing
Number of deposited models: 20 models
Backbone RMSD for the largest domain: 0.3 Å

Sample information

Author description:
SH3 domain of Hypothetical protein BAA76854.1
Source organism: Homo sapiens
Expression system: Not provided

Validation information

 
Structure ensemble information
Refinement method: torsion angle dynamics, simulated annealing
Number of calculated models: 100
Number of deposited models: 20 (target function)
Well-defined residues: A:6-A:77
Backbone RMSD for the largest domain: 0.3 Å
Representative model: 8 (medoid: most similar to other models)

Experimental information

Sample 1
Contents: 1.0mM PROTEIN U-15N, 13C; 20mM Phosphate buffer Na; 100mM NaCl; 0.02% NaN3; 90% H2O, 10% D2O Recorded spectra: 3D_13C-separated_NOESY, 3D_15N-separated_NOESY

Samples and spectra
NMR spectrometers used
Bruker AVANCE 800 MHz